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KMID : 0624620080410050408
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BMB Reports
2008 Volume.41 No. 5 p.408 ~ p.413
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Human brain pyridoxal-5’-phosphate phosphatase (PLPP):protein transduction of PEP-1-PLPP into PC12 cells
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Lee Yeom-Pyo
Kim Dae-Won
Lee Min-Jung
Jeong Min-Seop
Kim So-Young
Lee Sun-Hwa
Jang Sang-Ho
Park Jin-Seu
Kang Tae-Cheon
Won Moo-Ho
Cho Sung-Woo
Kwon Oh-Shin
Eum Won-Sik
Choi Soo-Young
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Abstract
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Pyridoxal-5’-phosphate phosphatase (PLPP) catalyzes the dephosphorylation of pyridoxal-5’-phosphate (PLP). A human brain PLPP gene was fused with a PEP-1 peptide and produced a genetic in-frame PEP-1-PLPP fusion protein. The purified PEP-1-PLPP fusion protein was efficiently transduced into PC12 cells in a time- and dose-dependent manner when added exogenously to culture media. Once inside the cells, the transduced PEP-1-PLPP fusion protein was stable for 36 h. The concentration of PLP was markedly decreased by the addition of exogenous PEP-1-PLPP to media pretreated with the vitamin B6 precursors; pyridoxine, pyridoxal kinase and pyridoxine-5’-phosphate oxidase into cells. The results suggest that the transduction of the PEP-1-PLPP fusion protein can be one mode of PLP level regulation, and to replenish this enzyme in the various neurological disorders related to vitamin B6. [BMB reports 2008;41(5): 408-413]
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KEYWORD
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Human brain pyridoxal-5’-phosphate phosphatase (PLPP), PEP-1 peptide, Protein transduction, Protein therapy, Pyridoxal-5’-phosphate (PLP)
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